The spread of influenza A(H1N1)pdm09 in Victorian school children in 2009:iImplications for revised pandemic planning

Background Victoria was the first state in Australia to experience community transmission of influenza A(H1N1)pdm09. We undertook a descriptive epidemiological analysis of the first 1,000 notified cases to describe the epidemic associated with school children and explore implications for school closure and antiviral distribution policy in revised pandemic plans. Methods Records of the first 1,000 laboratory-confirmed cases of influenza A(H1N1)pdm09 notified to the Victorian Government Department of Health between 20 May and 5 June 2009 were extracted from the state’s notifiable infectious diseases database. Descriptive analyses were conducted on case demographics, symptoms, case treatment, prophylaxis of contacts and distribution of cases in schools. Results Two-thirds of the first 1,000 cases were school-aged (5–17 years) with cases in 203 schools, particularly along the north and western peripheries of the metropolitan area. Cases in one school accounted for nearly 8% of all cases but the school was not closed until nine days after symptom onset of the first identified case. Amongst all cases, cough (85%) was the most commonly reported symptom followed by fever (68%) although this was significantly higher in primary school children (76%). The risk of hospitalisation was 2%. The median time between illness onset and notification of laboratory confirmation was four days, with only 10% of cases notified within two days of onset and thus eligible for oseltamivir treatment. Nearly 6,000 contacts were followed up for prophylaxis. Conclusions With a generally mild clinical course and widespread transmission before its detection, limited and short-term school closures appeared to have minimal impact on influenza A(H1N1)pdm09 transmission. Antiviral treatment could rarely be delivered to cases within 48 hours of symptom onset. These scenarios and lessons learned from them need to be incorporated into revisions of pandemic plans

Pandemic (H1N1) 2009 influenza community transmission was established in one Australian state when the virus was first identified in North America

BACKGROUND In mid-June 2009 the State of Victoria in Australia appeared to have the highest notification rate of pandemic (H1N1) 2009 influenza in the world. We hypothesise that this was because community transmission of pandemic influenza was already well established in Victoria at the time testing for the novel virus commenced. In contrast, this was not true for the pandemic in other parts of Australia, including Western Australia (WA). METHODS We used data from detailed case follow-up of patients with confirmed infection in Victoria and WA to demonstrate the difference in the pandemic curve in two Australian states on opposite sides of the continent. We modelled the pandemic in both states, using a susceptible-infected-removed model with Bayesian inference accounting for imported cases. RESULTS Epidemic transmission occurred earlier in Victoria and later in WA. Only 5% of the first 100 Victorian cases were not locally acquired and three of these were brothers in one family. By contrast, 53% of the first 102 cases in WA were associated with importation from Victoria. Using plausible model input data, estimation of the effective reproductive number for the Victorian epidemic required us to invoke an earlier date for commencement of transmission to explain the observed data. This was not required in modelling the epidemic in WA. CONCLUSION Strong circumstantial evidence, supported by modelling, suggests community transmission of pandemic influenza was well established in Victoria, but not in WA, at the time testing for the novel virus commenced in Australia. The virus is likely to have entered Victoria and already become established around the time it was first identified in the US and Mexico

Ideology and disease identity : the politics of rickets, 1929-1982

How can we assess the reciprocal impacts of politics and medicine in the contemporary period? Using the example of rickets in twentieth century Britain, I will explore the ways in which a preventable, curable non-infectious disease came to have enormous political significance, first as a symbol of socioeconomic inequality, then as evidence of racial and ethnic health disparities. Between the 1920s and 1980s, clinicians, researchers, health workers, members of Parliament and later Britain's growing South Asian ethnic communities repeatedly confronted the British state with evidence of persistent nutritional deficiency among the British poor and British Asians. Drawing on bitter memories of the ‘Hungry Thirties’, postwar rickets—so often described as a ‘Victorian’ disease—became a high-profile sign of what was variously constructed as a failure of the Welfare State; or of the political parties charged with its protection; or of ethnically Asian migrants and their descendants to adapt to British life and norms. Here I will argue that rickets prompted such consternation not because of its severity, the cost of its treatment, or even its prevalence; but because of the ease with which it was politicised. I will explore the ways in which this condition was envisioned, defined and addressed as Britain moved from the postwar consensus to Thatcherism, and as Britain's diverse South Asian communities developed from migrant enclaves to settled multigenerational ethnic communities

Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody

<p>Abstract</p> <p>Background</p> <p>The form(s) of amyloid-β peptide (Aβ) associated with the pathology characteristic of Alzheimer's disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal Aβ accumulation is an issue of considerable controversy; even the existence of Aβ deposits within neurons has recently been challenged by Winton and co-workers. These authors purport that it is actually intraneuronal APP that is being detected by antibodies thought to be specific for Aβ. To further address this issue, an anti-Aβ antibody was developed (MOAB-2) that specifically detects Aβ, but not APP. This antibody allows for the further evaluation of the early accumulation of intraneuronal Aβ in transgenic mice with increased levels of human Aβ in 5xFAD and 3xTg mice.</p> <p>Results</p> <p>MOAB-2 (mouse IgG_2b) is a pan-specific, high-titer antibody to Aβ residues 1-4 as demonstrated by biochemical and immunohistochemical analyses (IHC), particularly compared to 6E10 (a commonly used commercial antibody to Aβ residues 3-8). MOAB-2 did not detect APP or APP-CTFs in cell culture media/lysates (HEK-APP_Sweor HEK-APP_Swe/BACE1) or in brain homogenates from transgenic mice expressing 5 familial AD (FAD) mutation (5xFAD mice). Using IHC on 5xFAD brain tissue, MOAB-2 immunoreactivity co-localized with C-terminal antibodies specific for Aβ40 and Aβ42. MOAB-2 did not co-localize with either N- or C-terminal antibodies to APP. In addition, no MOAB-2-immunreactivity was observed in the brains of 5xFAD/BACE^-/-mice, although significant amounts of APP were detected by N- and C-terminal antibodies to APP, as well as by 6E10. In both 5xFAD and 3xTg mouse brain tissue, MOAB-2 co-localized with cathepsin-D, a marker for acidic organelles, further evidence for intraneuronal Aβ, distinct from Aβ associated with the cell membrane. MOAB-2 demonstrated strong intraneuronal and extra-cellular immunoreactivity in 5xFAD and 3xTg mouse brain tissues.</p> <p>Conclusions</p> <p>Both intraneuronal Aβ accumulation and extracellular Aβ deposition was demonstrated in 5xFAD mice and 3xTg mice with MOAB-2, an antibody that will help differentiate intracellular Aβ from APP. However, further investigation is required to determine whether a molecular mechanism links the presence of intraneuronal Aβ with neurotoxicity. As well, understanding the relevance of these observations to human AD patients is critical.</p

The impact of trammel nets as an MPA soft bottom monitoring method

With the global increase of marine protected area (MPA) implementation, the need for monitoring and the evaluation of their effectiveness becomes ever more important. Currently there is a severe lack of information about the protection effects of soft-substrate ecosystems. While many different methods have been established for the monitoring of hard-substrate ecosystems, most of these minimally invasive methods prove ineffective for soft- bottom habitats. Information and quantification of the impact of monitoring methods is needed to provide decision makers with the necessary knowledge to choose appropriate and feasible monitoring methods. In this study we quantify the impact of experimental trammel net fishing as a monitoring method of the soft-substrate demersal fish community using the Arrábida MPA (Portugal) as a case study. Over the 14 biannual sampling campaigns (between 2010 and 2019) 21,873 individuals and 5.61 tonnes of fish were caught. The gear is highly effective with an average catch per unit effort higher than reported for commercial fisheries in adjacent areas. When excluding the pelagic species, mortality rates are 41.2% and 30.4% in numbers and biomass, respectively. Most of the dead individuals belong to small, non-protected species with relatively little commercial value while MPA conservation target groups such as Soleidae and Rajidae have high survival rates. Due to its low size- and species-selectivity and the high survival rate of protected valuable species, the trammel net experimental fishing proved to be an effective monitoring method for soft-substrate demersal fish communities. Given their relatively low impact on the local ecosystem experimental trammel nets are a good alternative for areas where nonextractive methods are not effective. Nevertheless, quantification of the impact of other monitoring methods is necessary to enable the determination of the methods with the lowest mortality and impact for future softsubstrate MPA monitoring.LIFE06 NAT/P/000192 FCT: UID/Multi/04326/2020; DL 57/2016/CP1361/CT0036; European Maritime and Fisheries Fund INFORBIOMARES: POSEUR-03-2215-FC-000047info:eu-repo/semantics/publishedVersio

The Spaceborne Imaging Spectrometer DESIS: Mission summary and potential for scientific developments

The DLR Earth Sensing Imaging Spectrometer (DESIS) is a spaceborne instrument installed and operated on the International Space Station (ISS). The German Aerospace Center (DLR) has developed the instrument and the full pre-processing chain up to L2A, while the US company Teledyne Brown Engineering (TBE) provided the Multi-User System for Earth Sensing (MUSES) platform and the infrastructure for operations and data tasking. DESIS is equipped with an on-board calibration unit and a rotating pointing mirror (POI). The POI can change the line of sight in the forward/backward direction (independently of the MUSES orientation), allowing the observation of the same area with different pointing angles within an overflight. About four years after the mission’s kick-off, the DESIS spectrometer was integrated into MUSES in August 2018, marking the start of the commissioning phase. The DESIS on-orbit functional tests were successful, and the DLR-built processing chain installed at DLR for scientific users and at Amazon Web Service for commercial users started to generate operational L1B, L1C and L2A DESIS products. In October 2019 the operational phase started the distribution of the data to scientific and commercial users. Since then, the instrument performance has been constantly evaluated. In a continuous monitoring process, the data quality is controlled and, if necessary, the calibration algorithms and tables are adjusted. This is essential for the later data application by scientists. In particular, the monitoring approaches emphasize the need for high and consistent data quality over long time periods. In autumn 2021, the first DESIS user workshop demonstrated the widespread use of DESIS data for topics like water and terrestrial resource monitoring, biodiversity and forest management. This presentation will give an overview of the DESIS mission, data quality, data access, and provides examples and perspectives on the scientific exploitation of the mission. The contribution for the CHIME mission is presented exemplarily for the CHIME test sites that are constantly observed by DESIS since 2020. DESIS data acquisition opportunities rely on the non-sun-synchronous ISS orbit, resulting in observation and illumination conditions difficult to reproduce. On the other hand, DESIS time series contain images of different day times, sensor incident angles as well as sun zenith angles and thus, can open up new opportunities for the monitoring of Earth system processes that have a daily variability such as photosynthesis. Finally, DESIS multitemporal data stacks can be an essential data base for algorithm and operational processor developments that shall be able to handle massive data amounts. The DESIS data archive is open for such research and developments and thus, is a valuable imaging spectroscopy data source

Drawing lines at the sand: evidence for functional vs. visual reef boundaries in temperate Marine Protected Areas.

Marine Protected Areas (MPAs) can either protect all seabed habitats within them or discrete features. If discrete features within the MPA are to be protected humans have to know where the boundaries are. In Lyme Bay, SW England a MPA excluded towed demersal fishing gear from 206 km(2) to protect rocky reef habitats and the associated species. The site comprised a mosaic of sedimentary and reef habitats and so 'non reef' habitat also benefited from the MPA. Following 3 years protection, video data showed that sessile Reef Associated Species (RAS) had colonised sedimentary habitat indicating that 'reef' was present. This suggested that the functional extent of the reef was potentially greater than its visual boundary. Feature based MPA management may not adequately protect targeted features, whereas site based management allows for shifting baselines and will be more effective at delivering ecosystem goods and services

Measurement of the cross-section and charge asymmetry of WW bosons produced in proton-proton collisions at s=8\sqrt{s}=8 TeV with the ATLAS detector

This paper presents measurements of the $W^+ \rightarrow \mu^+\nu$ and $W^- \rightarrow \mu^-\nu$ cross-sections and the associated charge asymmetry as a function of the absolute pseudorapidity of the decay muon. The data were collected in proton--proton collisions at a centre-of-mass energy of 8 TeV with the ATLAS experiment at the LHC and correspond to a total integrated luminosity of 20.2~\mbox{fb^{-1}}. The precision of the cross-section measurements varies between 0.8% to 1.5% as a function of the pseudorapidity, excluding the 1.9% uncertainty on the integrated luminosity. The charge asymmetry is measured with an uncertainty between 0.002 and 0.003. The results are compared with predictions based on next-to-next-to-leading-order calculations with various parton distribution functions and have the sensitivity to discriminate between them.Comment: 38 pages in total, author list starting page 22, 5 figures, 4 tables, submitted to EPJC. All figures including auxiliary figures are available at https://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/STDM-2017-13